Surfactant protein D (SP-D) is a glycoprotein (Mw = 43 kDa) with a collagen-like structure. SP-D belongs to the subgroup (colectin) of the C-type lectin, produced in alveolar type II epithelial cells. Human serum SP-D is high in patients with interstitial pneumonia, suggesting that it serves as a diagnostic marker for interstitial pneumonia. Yamasa Corporation developed and is manufacturing and distributing SP-D kit “Yamasa” EIA II, a human SP-D measurement kit, which has been widely employed for laboratory tests (marketing is contracted to Kyowa Medex Co., Ltd.).

In addition, Yamasa Corporation investigated the rat SP-D measurement system and developed rat SP-D measurement ELISA with a monoclonal anti-rat SP-D antibody1). This ELISA allows highly sensitive measurement of rat serum SP-D. In addition, the antibody used in the ELISA also reacts with mouse SP-D, allowing the measurement of mouse SP-D. Serum SP-D concentrations in rats and mice with bleomycin-induced pulmonary fibrosis were examined using the ELISA, demonstrating that serum SP-D levels were high in those with lung injuries2) (summarized in this reference). Rat/Mouse SP-D Kit YAMASA EIA was developed using the ELISA.

To elucidate the functions of SP-D in vivo, animal experiments are indispensable. This product that allows highly sensitive measurement of rat/mouse SP-D is useful to investigate SP-D. In addition, a study using rats and mice with bleomycin-induced lung fibrosis demonstrated that SP-D may serve as a serum marker to monitor lung injury, suggesting that our product may be useful for detecting lung injuries in various animal experiments.

Features of the kit

  • ELISA with a 96-well microtiter plate.
  • Rat SP-D levels can be measured in a range of 0.47-30 ng/mL.
  • Measurement time: primary reaction, 2 hours; secondary reaction, 1 hour: and color reaction, 20 minutes.
  • Rat and mouse SP-D can be measured.*1
  • SP-D in rat and mouse sera and bronchoalveolar lavage fluids can be measured.*2

*1: Mouse SP-D concentrations are equivalents of rat SP-D. For detailed information, see (1) of “Notes of measurement.”

*2: Dilution ratios vary with samples. For detailed information, see (2) of “Notes of measurement.”

Principles of measurement

Sandwich ELSIA with two types of mouse monoclonal anti-rat SP-D antibody

Product contents

– Product code: 80072
– Components:

Name Volume Quantity
(1) Antibody solid phase plate 96-well microtiter plate 1 plate
(2) SP-D standard solution 1 (SP-D concentration: 0.47 ng/mL) 0.5mL 1 vial
(3) SP-D standard solution 2 (SP-D concentration: 1.88 ng/mL) 0.5mL 1 vial
(4) SP-D standard solution 3 (SP-D concentration: 7.5 ng/mL) 0.5mL 1 vial
(5) SP-D standard solution 4 (SP-D concentration: 30 ng/mL) 0.5mL 1 vial
(6) Diluent 50mL 1 vial
(7) Washing stock 50mL 1 vial
(8) Enzyme-labeled antibody 0.15mL 1 vial
(9) Diluent for enzyme-labeled antibodies 15mL 1 vial
(10) Coloring solution A 0.5mL 1 vial
(11) Coloring solution B 11mL 1 vial
(12) Stop solution 11mL 1 vial

– Storage:2-8℃
– Shelf life: 24 months after the date of manufacture
– Suggested Retail Price:¥ 120,000

Measuring methods



ラット/マウス SP-Dキット「ヤマサ」EIA

Example of a standard curve
ラット/マウス SP-Dキット「ヤマサ」EIA

Notes of measurement

(1) Since the standard solution attached to the product is rat SP-D, the measured values of mouse samples are calculated as equivalents of rat SP-D.

(2) Dilute samples with the attached diluent before measurement (dilution rates: 50-fold for rat sera, 10-fold for mouse sera, and 100-fold for bronchoalveolar lavage fluids). Dilute samples appropriately when their concentrations are above the measurement range.

(3) For more information, contact Marketing & MR Section.


1. Murata M et al. (2010). Exp Lung Res. 36(8). 463-468.
2. Murata M. et al.: J Toxicol Sci. Vol. 35:Supplement P.S 208
3. Murata M et al. (2016). Exp Lung Res. 42(6). 314-321.