Yamasa Corporation

The product contains all the necessary reagents. For preparation, stock solutions (10-fold concentrated solutions) are diluted with purified water. In addition, general-purpose equipment, such as a micropipette, and a microplate reader capable of measuring absorbance at 450 nm are needed.

The product contains all the necessary reagents. For preparation, stock solutions (10-fold concentrated solutions) are diluted with purified water. In addition, general-purpose equipment, such as a micropipette, and a microplate reader capable of measuring absorbance at 450 nm are needed.

Monoclonal antibodies used in this product react specifically with mouse IgE. Thus, IgE derived from other animal species cannot be measured.

We have not measured IgE values in all mouse species. The monoclonal antibodies used for this product react specifically with a commercially available mouse IgE. However, whether or not they react with any IgE in mouse sera remains unknown.

We have not measured IgE values in all mouse species. The monoclonal antibodies used for this product react specifically with a commercially available mouse IgE. However, whether or not they react with any IgE in mouse sera remains unknown.

As we investigated, IgE values were below the detection limit in most of BALB/c mice reared in SPF environments. Similar results may be obtained for Nc/Nga mice as far as they have no symptom (e.g., infection and inflammation), although not all mouse species have been examined. In addition, low IgE concentrations in samples may fall below the detection limit after dilution, as described below.

The lowest detection sensitivity of this product is about 2 ng/mL. Thus, no IgE may be detected in samples with ≤50 ng/mL IgE, because they are 25-fold diluted. Therefore, non-detection of IgE is not necessarily a failure of the product or any problem in measurement. Please check the absorbance of a standard solution used as a control.

This product is an ELISA kit using monoclonal antibodies, allowing the reaction and detection of mouse IgE if any in a sample. However, the effects of contained solvents (plasma components, medium components, buffer, etc.) should be examined. Thus, measurement should be conducted with a sample solvent supplemented with IgE at a known concentration to examine the effects of the solvent based on the recovery rate. As described above, IgE may fall below the detection limit if contained at low concentrations.

This product is an ELISA kit using monoclonal antibodies, allowing the reaction and detection of mouse IgE if any in a sample. However, the effects of contained solvents (plasma components, medium components, buffer, etc.) should be examined. Thus, measurement should be conducted with a sample solvent supplemented with IgE at a known concentration to examine the effects of the solvent based on the recovery rate. As described above, IgE may fall below the detection limit if contained at low concentrations.

We have no data available.

The effects of serum components preclude accurate measurements. The effects vary with samples, and can be ignored at a dilution factor of 25.

The product performance is guaranteed only when measurement is conducted using the regimens described in the package insert. Measurements, conducted otherwise, cannot be guaranteed.

The product performance is guaranteed only when measurement is conducted using the regimens described in the package insert. Measurements, conducted otherwise, cannot be guaranteed.

No, it cannot be measured only with the kit. In addition, we have not examined measurement using the kit. Please consider combinations with commercially available antibody reagents.

Absorbance varies with measurement conditions, even if samples of the same concentration are used. Thus, the standard solution and blank should be included in all measurements.

Measured values are concentrations in diluted samples, and, therefore, need to be multiplied by a dilution factor to determine sample concentrations. For 25-fold dilution, measured values need to be multiplied by 25. For >25-fold dilutions, apply the multiplying factors.

Measured values are concentrations in diluted samples, and, therefore, need to be multiplied by a dilution factor to determine sample concentrations. For 25-fold dilution, measured values need to be multiplied by 25. For >25-fold dilutions, apply the multiplying factors.

The product contains all the necessary reagents. For reagent preparation, stock solutions for washing and enzyme-labeled antibodies are diluted, and coloring reagents A and B are mixed. In addition, general-purpose equipment, such as a micropipette, and a microplate reader capable of measuring absorbance at 450 nm are needed.
The sample must be diluted, but no pretreatment is needed.

The product contains all the necessary reagents. For reagent preparation, stock solutions for washing and enzyme-labeled antibodies are diluted, and coloring reagents A and B are mixed. In addition, general-purpose equipment, such as a micropipette, and a microplate reader capable of measuring absorbance at 450 nm are needed.
The sample must be diluted, but no pretreatment is needed.

The product contains all the necessary reagents. For reagent preparation, stock solutions for washing and enzyme-labeled antibodies are diluted, and coloring reagents A and B are mixed. In addition, general-purpose equipment, such as a micropipette, and a microplate reader capable of measuring absorbance at 450 nm are needed.
The sample must be diluted, but no pretreatment is needed.

The performance of this product is guaranteed only when measurement is conducted using the reagents enclosed in the product as described in the package insert. Measured results cannot be guaranteed if obtained otherwise.

The performance of this product is guaranteed only when measurement is conducted using the reagents enclosed in the product as described in the package insert. Measured results cannot be guaranteed if obtained otherwise.

The performance of this product is guaranteed only when measurement is conducted using the reagents enclosed in the product as described in the package insert. Measured results cannot be guaranteed if obtained otherwise.

No, the standard solution and blank should be simultaneously measured every time, because absorbance varies with measurement conditions, even if the standard solution, blank, and samples of the same concentration are measured.
Since a standard curve varies with absorbance at each point of the standard solution, measurements need to be conducted at each point.

No, the standard solution and blank should be simultaneously measured every time, because absorbance varies with measurement conditions, even if the standard solution, blank, and samples of the same concentration are measured.
Since a standard curve varies with absorbance at each point of the standard solution, measurements need to be conducted at each point.

No, the standard solution and blank should be simultaneously measured every time, because absorbance varies with measurement conditions, even if the standard solution, blank, and samples of the same concentration are measured.
Since a standard curve varies with absorbance at each point of the standard solution, measurements need to be conducted at each point.

Since the standard solution is supplied in a form suitable for measurement, it cannot be used for other purposes. Rat SP-D protein is not supplied separately.

Since the standard solution is supplied in a form suitable for measurement, it cannot be used for other purposes. Rat SP-D protein is not supplied separately.

There is no method to calculate the amount of mouse SP-D from rat SP-D equivalent. We do not supply a standard solution for mouse SP-D.

There is no method to calculate the amount of mouse SP-D from rat SP-D equivalent. We do not supply a standard solution for mouse SP-D.

The reagents enclosed in the product are supplied in a form suitable for measurement. Thus, they cannot be used for other purposes.

Using the product, SP-D levels can be measured only in rats and mice. Whether or not SP-D levels can be measured in other animal species remain unknown.

SP-D levels have not been extensively examined in all rat/mouse species.

Considering the measurement principle, rat/mouse SP-D may be detected in various samples under conditions that allow antibody reactions. However, it may not be accurately measured, because the effects of other components and solution conditions have not been examined.
The reactivity of antibodies may be altered by amino acid sequences and three-dimensional structures that differ between naturally-occurring and recombinant proteins. Thus, recombinant proteins may not be measured in the same manner as native forms.
This product is intended for the measurement of SP-D in sera and bronchoalveolar lavage fluids. Thus, measurements in other samples cannot be guaranteed.

We have not examined the recognition site of the antibody.

Considering the measurement principle, rat/mouse SP-D may be detected in various samples under conditions that allow antibody reactions. However, it may not be accurately measured, because the effects of other components and solution conditions have not been examined.
The reactivity of antibodies may be altered by amino acid sequences and three-dimensional structures that differ between naturally-occurring and recombinant proteins. Thus, recombinant proteins may not be measured in the same manner as native forms.
This product is intended for the measurement of SP-D in sera and bronchoalveolar lavage fluids. Thus, measurements in other samples cannot be guaranteed.

Samples, diluted at a lower dilution ratio than that described in the package insert, may not be accurately measured because of the effects of other components in the sample. Thus, measurement needs to be conducted at the dilution ratio described in the package insert.

Normal values have not been determined in rats and mice using the product. In addition, SP-D value may vary with breeding and experimental conditions. Be sure to prepare control samples for each test. Measurements with ELISA, a prototype of this product, in normal rats, can be seen here (Note: These are reference values, because they were obtained using a prototype with small sample size.).

No report has been published on elevated SP-D values in rats and mice. However, reportedly, SP-D values are elevated by interstitial pneumonia, hypersensitive pneumonitis, and lung cancer lines in humans.