Rat/mouse OCT assay kit
Non-alcoholic steatohepatitis (NASH) and alcoholic liver disease (ALD) show similar pathological findings, with the involvement of mitochondrial disorders1). Therefore, Yamasa Corporation investigated ornithine carbamoyltransferase (OCT), localized in liver mitochondria, as a marker of liver mitochondrial disorders, and developed rat/mouse OCT quantitative ELISA using a monoclonal antibody. Blood OCT concentrations in rat models of liver injury were examined with the ELISA, demonstrating that OCT was more useful than cytoplasmic markers, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), in rat models of drug-induced liver injury 2)3), NASH 4) and ALD4).
A rat/mouse OCT assay kit, developed based on the ELISA, can be employed for quantifying rat and mouse serum OCT concentrations.
– ELISA with a 96-well microtiter plate.
– Measurement time: primary reaction, 1 hour; secondary reaction, 2 hours; and color reaction, 30 minutes
– Rat and mouse*1 serum OCT concentrations can be measured.*2
*1: Rat OCT concentrations were used as standards. Mouse OCT concentrations were calculated as equivalents to the rat OCT concentrations.
*2: Samples need to be diluted before measurement. For details, see (2) of “Notes of measurement.”
Sandwich ELSIA was developed using two kinds of mouse anti-OCT monoclonal antibody.
– Product code: 80127
|(1)||Antibody Coated Plate||96-well microwells plate||1 plate|
|(2)||OCT Standard 1 (0ng/mL)||0.05mL||1 vial|
|(3)||OCT Standard 2 (30ng/mL)||0.05mL||1 vial|
|(4)||OCT Standard 3 (100ng/mL)||0.05mL||1 vial|
|(5)||OCT Standard 4 (300ng/mL)||0.05mL||1 vial|
|(7)||Concentrated Washing Solution||50mL||1 vial|
|(8)||Enzyme Conjugate||0.15mL||1 vial|
|(9)||Color Developing Reagent A||6mL||1 vial|
|(10)||Color Developing Reagent B||6mL||1 vial|
|(11)||Stop Solution||11mL||1 vial|
– Storage: 2-8℃
– Shelf life: 6 months after the date of manufacture
– Suggested retail price: ¥ 143,000
(1) Serums of rat or muse are diluted 11-fold with Diluent. If the OCT level of the sample exceed measuring range, dilute with Diluent to obtain a value within the range..
(2) For more information, contact Marketing & MR Section.
1. Pessayre D. et al. (2005). J Hepatol. 42. 928-40.
2. Murayama H. et al. (2007). Clin Chim Acta. 375. 63-68.
3. Murayama H. et al. (2008). Clin Chim Acta. 391. 31-35.
4. Murayama H. et al. :The 36th Annual Meeting of The Japanese Society of Toxicology
5. Furihata T et al. (2016). Drug Metab Pharmacokinet. 31. 102-105.