MOUSE IgE EIA KIT YAMASA
This ELISA kit was developed with two kinds of monoclonal anti-mouse IgE antibody. Mouse IgE can be measured with high sensitivity. The antibodies are highly specific to mouse IgE and do not cross-reactive with other immunoglobulins. All reagents are enclosed in the kit, eliminating the need of new reagent preparation. The reaction is completed in about 2 hours. A 96-well microtiter plate allows simultaneous measurement of many samples. Only a microplate reader capable of measuring absorbance at 450 nm is needed, and no other special equipment is needed.
– Product code: 7675
– Kit contents
|(1) Mouse IgE plate||96-well microtiter plate (8 wells x 12)||1 set|
|(2) Mouse IgE antibody enzyme solution||11mL||1 bottle|
|(3) Mouse IgE standard solutions
500, 250, 100, 50, and 10 ng/mL
|1mL||1 bottle each|
|(4) Sample diluent (0 ng/mL standard solution)||30mL||1 bottle|
|(5) Coloring solution||15mL||1 bottle|
|(6) Washing stock solution (10x concentrated)||50mL||1 bottle|
|(7) Reaction stop solution||12mL||1 bottle|
○ Storage: 2-8℃.
○ Shelf life: Indicated outside the box.
|Dilute mouse sera 25-fold* before measurement.
*Note: Sera at a higher concentration may influence the measurement.
|1.||Set a mouse IgE plate in the well holder|
|2.||Add 100 μL each of diluted sample, mouse IgE standard solution, and sample diluent.|
|3.||Incubate the sample at 20-25℃ for 30 minutes.|
|4.||Wash the plate with 300 μL of washing solution three times.|
|5.||Add 100 μL of mouse IgE antibody enzyme solution.|
|6.||Incubate the sample at 20-25℃ for 30 minutes.|
|7.||Wash the plate under the same conditions as 4.|
|8.||Add 100 μL of coloring solution.|
|9.||Incubate the sample at 20-25℃ for 15 minutes.|
|10.||Add 100 μL of reaction stop solution.|
|11.||Measure absorbance at 450 nm.|
|12.||Read the mouse IgE concentration in the sample using a standard curve.|
|[Example of standard curve]
||[Example of mouse serum measurement]
Serum IgE concentrations were measured in Nc/Nga mice with dermatitis using the kit.
1. T. Hirano et al. (1988). Int archs allergy appl. immun. 85. 47.
2. T. Hirano et al. (1988). Medical immunology 15. 11.