MOUSE IgE EIA KIT YAMASA

Features

This ELISA kit was developed with two kinds of monoclonal anti-mouse IgE antibody. Mouse IgE can be measured with high sensitivity. The antibodies are highly specific to mouse IgE and do not cross-reactive with other immunoglobulins. All reagents are enclosed in the kit, eliminating the need of new reagent preparation. The reaction is completed in about 2 hours. A 96-well microtiter plate allows simultaneous measurement of many samples. Only a microplate reader capable of measuring absorbance at 450 nm is needed, and no other special equipment is needed.

Contents

– Product code: 7675
– Kit contents

Reagents Volume Quantity
(1) Mouse IgE plate 96-well microtiter plate (8 wells x 12) 1 set
(2) Mouse IgE antibody enzyme solution 11mL 1 bottle
(3) Mouse IgE standard solutions
500, 250, 100, 50, and 10 ng/mL		 1mL 1 bottle each
(4) Sample diluent (0 ng/mL standard solution) 30mL 1 bottle
(5) Coloring solution 15mL 1 bottle
(6) Washing stock solution (10x concentrated) 50mL 1 bottle
(7) Reaction stop solution 12mL 1 bottle

○ Storage: 2-8℃.
○ Shelf life: Indicated outside the box.

 

Outline of measurement

Dilute mouse sera 25-fold* before measurement.
*Note: Sera at a higher concentration may influence the measurement.
1. Set a mouse IgE plate in the well holder
2. Add 100 μL each of diluted sample, mouse IgE standard solution, and sample diluent.
3. Incubate the sample at 20-25℃ for 30 minutes.
4. Wash the plate with 300 μL of washing solution three times.
5. Add 100 μL of mouse IgE antibody enzyme solution.
6. Incubate the sample at 20-25℃ for 30 minutes.
7. Wash the plate under the same conditions as 4.
8. Add 100 μL of coloring solution.
9. Incubate the sample at 20-25℃ for 15 minutes.
10. Add 100 μL of reaction stop solution.
11. Measure absorbance at 450 nm.
12. Read the mouse IgE concentration in the sample using a standard curve.

Measured values

 

[Example of standard curve]
マウスIgE測定キット「ヤマサ」EIA		 [Example of mouse serum measurement]
Serum IgE concentrations were measured in Nc/Nga mice with dermatitis using the kit.

Sample Concentration (ng/mL)
1 1212 Mean ± SD = 4211 ± 1,985 ng/mL

Note: No IgE was detected in the 25-fold diluted sera of SPF BALB/c mice.
2 4672
3 6264
4 2827
5 3386
6 2849
7 4810
8 7607
9 5926
10 2556

For inquiries, see Q&A or contact the Marketing & MR Section.

References

1. T. Hirano et al. (1988). Int archs allergy appl. immun. 85. 47.
2. T. Hirano et al. (1988). Medical immunology 15. 11.