Yamasa Corporation

Immunoassay using the set

１．soluble Human CD23(soluble Fcε receptorII)
sCD23 can be measured in a range of 6.25-400 units/mL.
<Products>

Code	Intended use
7591 7596(BER-02) 7597(BER-04-HRP)	Standard solution Solid phase antibody Labeled antibody

An example of sCD23 measurement by ELISA using the above three products.

 

2. Rat IgE
Various rat serum IgE antibodies can be measured.

Code	Intended use
7592 7610	Solid phase antibody Labeled antibody

Dilute commercially available rat IgE, as needed, to be used as a standard solution.
An example of rat IgE measurement by ELISA using the above two products.

<Results>

3. Endothelin
Human ET-1, -2, and bET-1 can be measured.
<Products>

Code	Intended use
7631(ET-01) 7632(ET-02)	Solid phase antibody Labeled antibody

Use endothelin of interest as a standard solution.
Couple 7632 to HRP by a conventional method to be used as a labeled antibody.

<Example of measurement>
An example of endothelin measurement by ELISA using the above two products.
Label 7632 with HRP by Nakane’s method to be used as a labeled antibody.

<Results>

4. Rat BGP
BGP concentrations in various rat sera can be measured.
<Products>

Code	Intended use
7609 7613 7614	Standard solution Solid phase antibody Labeled antibody

<Example of measurement>
An example of RBGP measurement by ELISA using the above three products.

<Example of BGP measurement in various rat sera >
An example of BGP measurement in various rat sera using the above method is shown.

Rat strains	n=	Concentration (ng/mL) Mean ± SD
Wistar　Rat	5	60±4.4
SD Rat	5	34±2.1
SHR　Rat	5	72±7.4

5. Mouse albumin

Mouse albumin can be measured.
<Products>

Code	Intended use
7618 7969	Solid phase antibody Labeled antibody

For the development of the product, purified mouse albumin was commercially available as a standard solution.
However, its sales are currently suspended, making the purified product difficult to obtain.
Hence, to conduct EIA for quantification as described below, albumin needs to be purified from mouse sera.
To quantify the increase or decrease in concentrations using the same experimental system, the mouse sera need to be diluted as needed to adjust absorbance to be used as a reference.
It should be noted that we neither provide a purified standard nor receive a contract of purification.

<Example of use>
An example of EIA measurement using the above products.

Add 100ul/well of 10ug/ml 7618 to microtiter plate. Incubate at 4C for overnight and block with 0.5% Skim milk/PBS for 1 hour. Shake out the solution. Add 100u1 of sample diluted by 1%BSA/PBS. Incubate for 1 hour at room temperature. Wash 3 times 0.05% Tween 20 in PBS. Add 100u1 of 7969 diluted x100 with 1%BSA/PBS. Incubate for 1 hour at room temperature. Wash 3 times. Add 100 ul of TMBZ solution. Incubate to develop the color. Add 100u1 of 1N H2SO4. Read at 450 nm.

6. Mouse transferrin

Mouse transferrin can be measured.

<Products>

Code	Intended use
7619 7970	Solid phase antibody Labeled antibody

Dilute commercially available mouse transferrin, as needed, to be used as a standard solution.

<Example of use>
An example of EIA measurement using the above products.

7. Mouse hemoglobin

Mouse hemoglobin can be measured.

<Products>

Code	Intended use
7575 7971	Solid phase antibody Labeled antibody

Dilute commercially available mouse hemoglobin, as needed, to be used as a standard solution.

< An example of EIA measurement using the above products.

Add 100ul/well of 10ug/ml 7575 to microtiter plate. Incubate at 4C for overnight and block with 0.5% Skim milk/PBS for 1 hour. Shake out the solution. Add 100u1 of sample diluted by 1%BSA/PBS. Incubate for 1 hour at room temperature. Wash 3 times 0.05% Tween 20 in PBS. Add 100u1 of 7971 diluted x100 with 1%BSA/PBS.. Incubate for 1 hour at room temperature. Wash 3 times. Add 100 ul of TMBZ solution. Incubate to develop the color. Add 100u1 of 1N H2SO4. Read at 450 nm.

 

Cross-reactivity

(1) Anti-muscle myosin heavy chain antibody
Reactivity of anti-SM1, -SM2, and SMemb antibodies to antigens of various rat strains is shown below.
No animals other than those listed in the table were examined.

(2) Anti-interferon antibody
Anti-INF antibodies against α and β subunits (human and mouse antigens) are commercially available.
Cross-reactivity of each antibody is shown below.

(3) Anti-mouse immunoglobulin antibody
Various anti-mouse immunoglobulin antibodies are commercially available.
Cross-reactivity of each antibody is shown below.